Iranian Journal of Colorectal Research

Iranian Journal of Colorectal Research

Anti-proliferative and Apoptotic Potential of Ferula Asafoetida’s Essential Oil on Colon Cancer Cell Lines Via Inhibiting NF-κB and TNF-alpha Receptors Pathway

Document Type : Research/Original Article

Authors
Shailja Verma 1, 2
Sanjay Joshi 2
Vijay Kothari 1
Sriram Seshadri 1
1 Institute of Science, Nirma University, Ahmedabad, India
2 Department of Research and Development, National Foods, The Hing Research Center, GIDC-Waghodia, Vadodara, India
10.30476/acrr.2023.97939.1174
Abstract
Background: The current investigation was conducted with the aim to elucidate and determine the anti-cancer efficacy in vitro on colon carcinoma human cell lines and to determine its probable mode of action. 
Methods: Anti-proliferative and apoptotic effect of various doses of essential oil from Ferula asafoetida was investigated on colon cancer cell lines i.e. CW620 and CT26. WT. The percentage cytotoxicity was determined by MTT assay. In addition, expression studies were carried out to determine the mode of action by determining the levels of NF-kB, TNF-alpha, TGF-beta and Caspases. 
Results: The potent anti-proliferative activity has been observed in both the cell lines. The percentage cytotoxicity is directly proportional to the increasing concentration of the essential oil. The same was also confirmed in the expression studies which shows significant upregulation of the antiapoptotic characteristics and down regulation of pro-carcinogenic factors. 
Conclusion: The essential oil of Ferula asafoetida shows potent anti-cancer activity and shows the role in gene regulation to attenuate the colon cancer.
Keywords
Colon cancer
Ferula asafoetida
Essential oil
Caspases
Inflammation
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Iranian Journal of Colorectal Research
Volume 11, Issue 1
Winter 2023
Pages 15-21